Complete Event Capture · Glioma TMA
Every QuPath action.
All in one passport.
Every action type that qTrace records — all converging into a single .qtrace passport per image. ⏺ REC on each step.
Main workflow steps
Core_01 · H&E
Annotation
neuropathologist · QuPath ROIs · author tracked
REC
Core_01 · Xenium
Cell Detection / Segmentation
StarDist · watershed · parameters + script
REC
Core_01 · DAPI / GFP
Image Alignment
AffineServer · Warpy · transform matrix 6×
REC
Additional captured events
Pixel Classifier
training + applying · SHA-256 + Git hash · channel config · fidelity
REC
Cell Type Classification
object classifiers · RCTD labels · applied_at + applied_by
REC
Cell Intensity Classification
Ki-67 · DAB OD mean · thresholds [+1, +2, +3]
REC
Multi-modal Overlay
MSI + Xenium · co-registered modalities · overlay step
REC
TMA De-Array
core extraction · grid coordinates · core labels
REC
Groovy Replay Script
deduplicated · committed to embedded Git · reproducible
REC
Validation Stamp
validator · scope · confidence · classifier_fidelity
REC
1 passport
per image
per image
.qtrace Passports
📄 Core_01.qtrace
image · session · alignment · steps · classifiers · cell_intensity · annotations · validation
📄 Core_02.qtrace
📄 Core_03.qtrace
↕ 20 passports · 20 TMA cores
Cohort TrackerReal-time QA status · filter by contributor, class, validation state, fidelity
Ready to add this layer of QA to your QuPath workflows?